The structural rearrangement of testicular arteries in case of chronic ethanol intoxication
DOI:
https://doi.org/10.15574/PS.2023.81.81Keywords:
morphometry, testis, testicular arteries, ethanol intoxicationAbstract
Prolonged poisoning of the body with ethanol damages almost all its organs and systems. Structural changes in testicular arteries under the influence of chronic ethanol intoxication have not been fully studied.
Purpose - to analyze the morphometric parameters of the small caliber arteries of the right and left testicles of rats in case of chronic ethanol intoxication.
Materials and methods. 62 laboratory sexually mature male rats was performed. The animals were divided into two groups. The Group 1 (control) included 30 rats, the Group 2 (experimental) - 32 animals. The experimental rats, which were conducted to the experiment, were injected intragastrically with a 30% ethanol solution at the rate of 2 ml per 100 g of body weight for 28 days once a day. Animals were euthanized by heart bleeding under thiopental anesthesia. Histological micro-sections were made from the left and right testicles. Morphometrically, the outer and inner diameters of the arteries, media thickness, adventitia, Vogenvoort and Kernogan indices, the height of endotheliocytes, the diameter of their nuclei, the nuclear-cytoplasmic ratio in these cells, and the relative volume of damaged endotheliocytes were determined morphometrically in small-caliber arteries. Statistical processing of digital data was carried out using Excel (Microsoft, USA) and STATISTICA 6.0 (Statsoft, USA) software.
Results. It was established that in case of simulated experimental conditions of chronic ethanol intoxication, the investigated morphometric parameters of the arteries of the left and right testicles changed significantly. In case of chronic intoxication with ethanol in rats, pronounced structural rearrangement of testicular vessels, mainly small arteries, characterized by thickening of the blood vessel’s wall, narrowing of vessels’ lumen, rearrangement of endothelial cells, and an increase in the relative volume of the damaged cells of intima, is established, and is dominant in the left testis.
Conclusions. In case of chronic intoxication with ethanol the structural changes in testicular tissues are dominant in the left testis. Pronounced remodeling of small caliber testicular arteries causes endothelial dysfunction, deterioration of blood supply to the testicles, hypoxia, dystrophic and necrobiotic tissue changes, infiltrative and sclerotic processes, which are the pathomorphological basis of male infertility.
When carrying out experiments with laboratory animals, all bioethical norms and recommendations were observed.
No conflict of interests was declared by the authors.
References
Adaramoye OA, Arisekola M. (2013). Kolaviron, a biflavonoid complex from Garcinia kola seeds, ameliorates ethanol-induced reproductive toxicity in male wistar rats. Niger J Physiol Sci. 28 (1): 9-15.
Akinloye DI, Ugbaja RN, Adebiyi AA et al. (2022). Duration effects of alcohol graded concentrations on the extent of lipid peroxidation, testis morphology and sperm quality assessment in Wistar rats. Toxicol Rep. 9: 1082-1091. https://doi.org/10.1016/j.toxrep.2022.05.003; PMid:36518383 PMCid:PMC9742911
Bahrii MM, Dibrova VA, Popadynets OH et al. (2016). Metodyky morfolohichnykh doslidzhen. Vinnytsia: Nova knyha: 328.
Condorelli RA, Calogero AE, Vicari E et al. (2015). Chronic consumption of alcohol and sperm parameters: our experience and the main evidences. Andrologia. 47: 368-379. https://doi.org/10.1111/and.12284; PMid:24766499
Council of Europe Treaty Series - Explanatory Reports. (1986). European convention for the protection of vertebrate animals used for experimental and other scientific purposes. Council of Europe. Strasbourg.
Denefil OV, Bilyk YaO, Chorniy SV, Fedoniuk LYa, Chorniі NV. (2022). The peculiarities of morpological changes of rats' ovary and biochemical state under the damage with different doses of lead acetate. Vidomosti likarski. 2 (75): 377-382. https://doi.org/10.36740/WLek202202109; PMid:35307662
Dugum M, McCullough A. (2015). Diagnosis and management of alcoholic liver disease. J. Clin. Transl. Hepatol. 3: 109-116.
Fedoniuk LYa, Nesteruk SO, Hnatiuk MS, Smachylo II, Tverdochlib VV, Yakymchuk OA. (2023). Quantitative morphological featuresbof the structural rearrangement of the venous blood vessels of the prostate gland in post-resection portal hypertension. Pol Merkur Lek. LI. 6: 608-612. https://doi.org/10.36740/Merkur202306105; PMid:38207061
Feldman HS, Jones KL, Lindsay S, Slymen D, Klonoff-Cohen H, Kao K et al. (2012, Apr). Prenatal alcohol exposure patterns and alcohol-related birth defects and growth deficiencies: a prospective study. Alcohol Clin Exp Res. 36 (4): 670-676. Epub 2012 Jan 17. https://doi.org/10.1111/j.1530-0277.2011.01664.x; PMid:22250768
Graff-Iversen S, Jansen MD, Hoff DA et al. (2013). Divergent associations of drinking frequency and binge consumption of alcohol with mortality within the same cohort. J. Epidemiol. Community Health. 67: 350-357. https://doi.org/10.1136/jech-2012-201564; PMid:23235547
Grover S, Mattoo SK, Pendharkar S et al. (2014). Sexual dysfunction in patients with alcohol and opioid dependence. Indian J Psychol Med. 36: 355-365. https://doi.org/10.4103/0253-7176.140699; PMid:25336765 PMCid:PMC4201785
Guthauser B, Boitrelle F, Plat A et al. (2014). Chronic excessive alcohol consumption and male fertility: a case report on reversible azoospermia and a literature review. Alcohol Alcohol. 49: 42-44. https://doi.org/10.1093/alcalc/agt133; PMid:23969551
Herman OM, Herasymiuk IYe, Fedoniuk LYa. (2021). Character and specifics of the structural alteration of the parenchyma and bloodstream of the testes of white rats with prolonged administration of high doses of prednisolone. Vidomosti likarski. 12 (74): 31473151. https://doi.org/10.36740/WLek202112104; PMid:35058380
Hnatiuk MS, Bodnarchuk IV, Tatarchuk LV. (2019). Morphometric evaluation of the features of the structural rearrangement of the hemomicrocirculatory bed of the tongue in desquamative glossitis. Bulletin of problems biology and medicine. 1: 88-92. https://doi.org/10.29254/2077-4214-2019-2-1-150-243-245
Jin B, Sun D, Dong W et al. (2020). Capsule Can Improve the Function of the Testicular Angiogenesis through Activating VEGFA/eNOS Signaling Pathway. Evid Based Complement Alternat Med: 1957267. https://doi.org/10.1155/2020/1957267; PMid:32382279 PMCid:PMC7197003
Kovalev GA, Petrenko AYu. (2004). Experimental model of alcoholic liver damage in female rats. Bulletin of Kharkiv National University. 617: 15-18.
Maneesh M, Jayalekshmi H, Dutta S et al. (2005). Effect of chronic ethanol administration on testicular antioxidant system and steroidogenic enzyme activity in rats. Indian J Exp Biol. 43 (5): 445-449.
Martinez FE, Martinez M, Padovani CR et al. (2000). Morphology of testis and epididymis in an ethanol-drinking rat strain (UChA and UChB). J Submicrosc Cytol Pathol. 32 (2): 175-184.
National Institute on Alcohol Abuse and Alcoholism. (2022). Alcohol. Facts and Statistics. URL: https://www.niaaa.nih.gov/publications/brochures-and-fact-sheets/alcohol-facts-and-statistics.
Osowski A, Fedoniuk L, Bilyk Ya, Fedchyshyn O, Sas M, Kramar S et al. (2023). Lead Exposure Assessment and Its Impact on the Structural Organization and Morphological Pecularities of Rat Ovaries. Toxics. 11: 9. https://doi.org/10.3390/toxics11090769; PMid:37755779 PMCid:PMC10536919
Oyedokun PA, Akhigbe RE, Ajayi LO et al. (2023). Impact of hypoxia on male reproductive functions. Mol Cell Biochem. 478 (4): 875-885. https://doi.org/10.1007/s11010-022-04559-1; PMid:36107286
Pendharkar S, Mattoo SK, Grover S. (2016). Sexual dysfunctions in alcohol-dependent men: a study from north India. Indian J Med Res. 144: 393-399. https://doi.org/10.4103/0971-5916.198681; PMid:28139538 PMCid:PMC5320845
Rissato JH, Ietsugu MV, Almeida CC et al. (2003). Morphology of the vas deferens in an ethanol-drinking strain of rats (UChA and UChB). J Submicrosc Cytol Pathol. 35 (3): 331-341.
Rivas I, Sanvisens A, Bolao F et al. (2013). Impact of medical comorbidity and risk of death in 680 patients with alcohol use disorders. Alcohol. Clin. Exp. Res. 37: E221-E227. https://doi.org/10.1111/j.1530-0277.2012.01861.x; PMid:23320801
Siervo GE, Vieira HR, Ogo FM et al. (2015). Spermatic and testicular damages in rats exposed to ethanol: influence of lipid peroxidation but not testosterone. Toxicology. 330: 1-8. https://doi.org/10.1016/j.tox.2015.01.016; PMid:25637669
Touvier M, Druesne-Pecollo N, Kesse-Guyot E et al. (2014). Demographic, socioeconomic, disease history, dietary and lifestyle cancer risk factors associated with alcohol consumption. Int. J. Cancer. 134: 445-459. https://doi.org/10.1002/ijc.28365; PMid:23824873
Uygur R, Yagmurca M, Alkoc OA et al. (2013). Effects of quercetin and fish n‐3 fatty acids on testicular injury induced by ethanol in rats. Andrologia. 46 (4): 356-369. https://doi.org/10.1111/and.12085; PMid:23488863
Van Heertum K, Rossi B. (2017). Alcohol and fertility: how much is too much? Fertil Res Pract. 3: 10. https://doi.org/10.1186/s40738-017-0037-x; PMid:28702207 PMCid:PMC5504800
World Health Organization. (2018). Global status report on alcohol and health 2018. Geneva: World Health Organization.
World Health Organization. (2018). Alcohol Key Facts. URL: https://www.who.int/news-room/fact-sheets/detail/alcohol.
Zamkevich VB, Diachuk MD, Gruzieva TS. (2019). The evaluation of alcohol consumption by the population and related problems. Clinical and preventive medicine. 3-4 (9-10): 93-99. https://doi.org/10.31612/2616-4868.4(10).2019.03
Zato'nski W, Mlozniak I, Zato'nski M et al. (2019). Small bottles - Huge problem? A new phase of Poland's ongoing alcohol epidemic. J. Health Inequalities. 5: 86-88. https://doi.org/10.5114/jhi.2019.87842
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Paediatric Surgery (Ukraine)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The policy of the Journal “PAEDIATRIC SURGERY. UKRAINE” is compatible with the vast majority of funders' of open access and self-archiving policies. The journal provides immediate open access route being convinced that everyone – not only scientists - can benefit from research results, and publishes articles exclusively under open access distribution, with a Creative Commons Attribution-Noncommercial 4.0 international license(СС BY-NC).
Authors transfer the copyright to the Journal “PAEDIATRIC SURGERY.UKRAINE” when the manuscript is accepted for publication. Authors declare that this manuscript has not been published nor is under simultaneous consideration for publication elsewhere. After publication, the articles become freely available on-line to the public.
Readers have the right to use, distribute, and reproduce articles in any medium, provided the articles and the journal are properly cited.
The use of published materials for commercial purposes is strongly prohibited.
