Probiotic disinfectants in the complex treatment of experimental peritonitis
DOI:
https://doi.org/10.15574/PS.2024.4(85).3142Keywords:
peritonitis, endogenous intoxication, multiple organ failure, probioticsAbstract
Aim - to investigate the effectiveness of probiotic disinfectants in the complex treatment of experimental peritonitis.
Materials and methods. The experimental study was performed on 45 white sexually mature rats, which were simulated with fecal peritonitis. The rats were divided into 3 groups of 15 animals. The treatment of rats in the group I (main) consisted of washing the abdominal cavity with a 5% solution of probiotic disinfectant, applying a probiotic disinfectant spray to the abdominal organs and a gel with probiotics to the surgical wound after its suturing. For the treatment of rats in thr group II (comparison group), a sorbent solution was used to wash the abdominal cavity. The treatment of rats in the group III (placebo control) consisted of washing the abdominal cavity with 0.9% NaCl. The content of medium-weight molecules (total), the toxin-binding capacity of serum proteins, and the functional activity of monocytes in the general NST test were studied. A pathomorphological study was also conducted.
Results. The use of the developed treatment in the experiment in animals of the group I contributed to a decrease in the content of medium-weight molecules and the restoration of the toxin-binding capacity of albumin already on the 5th day. These trends cause a decrease in the cytolytic activity of blood serum relative to its own leukocytes and the preservation of the functional capabilities of monocytes at an adapted level already on the 5th day. At the same time, on the 7th day, the indicators of the functional activity of monocytes corresponded to the reference values. In animals of the group II, the optimization of the studied indicators occurred only on the 7th day, but they did not correspond to the reference values. In the group III, the studied indicators were significantly reduced relative to the reference values throughout the study.
Conclusions. The results of the study demonstrate the effectiveness of using probiotic disinfectants for the sanitation of the abdominal cavity in animals with fecal peritonitis.
The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institution. When carrying out experiments with laboratory animals, all bioethical norms and recommendations were observed.
No conflict of interest was declared by the authors.
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